Migration and distribution of superparamagnetic iron oxide-labeled adipose-derived stem cells in the brain of cerebral infarction rats: MRI observation

doi:10.3969/j.issn.2095-4344.2016.06.005

Abstract

Abstract:

BACKGROUND: To trace the survival status of transplanted stem cells and the integration with host tissues using non-invasive imaging techniques are the focus of research in recent years.

OBJECTIVE: To observe the distribution and migration of superparamagnetic iron oxide (SPIO)-labeled adipose-derived stem cells in the brain of rats with cerebral infarction.

METHODS: Rat models of cerebral infarction were established and randomized into SPIO-labeled group and unlabeled group. At 1 day after modeling, the rats in the two groups were given SPIO-labeled adipose-derived stem cell suspension (10 μL) and unlabeled adipose-derived stem cell suspension (10 μL) into the brain, respectively. At 1, 7, 14 days after cell transplantation, neurological severity scores were measured, and MRI was used to observe the distribution of SPIO-labeled adipose-derived stem cells.

RESULTS AND CONCLUSION: At 7 and 14 days after transplantation, the neurological severity scores in the two groups were significantly lower than those at 1 day after transplantation (P < 0.05). However, there was no difference in the neurological severity scores between the two groups (P > 0.05). At 14 days after transplantation, MRI findings showed low signals in the transplanted region, indicating the cells migrated from the corpus callosum to the lesion. These findings suggest that intracerebral transplantation of adipose-derived stem cells can promote neurological recovery from cerebral infarction in rats, and MRI can be used to visualize the distribution and migration of SPIO-labeled adipose-derived stem cells in the brain.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Chen Long-hua. Migration and distribution of superparamagnetic iron oxide-labeled adipose-derived stem cells in the brain of cerebral infarction rats: MRI observation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 793-798.

Figures/Tables 5

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